18. GCP Comparison with Commercial Anti-angiogenesis Products:

Materials:

GCP, Product A （Containing 40 % isoflavone), Product B （Containing 40 % isoflavone aglycones), Powder shark cartilage (Containing 60% shark cartilage), Liquid shark cartilage

Preparation of samples：Dissolved in PBS or in DMSO by 100mg/ml concentration, and diluted with PBS, the final DMSO concentration is less than1％, 0.2ml of samples was added in the eggs. The liquid shark cartilage was added by original liquid. Each sample was added into 3 eggs.

Result:

Method：Tumor-bearing mice were transplanted with S-180 sarcoma. GCP, Product A, & Product B were dissolved to in tap water to create a 10% solution and administrated, p.o. in the dosage of 0.35ml/day/mouse (1g/kg body weight). The liquid shark cartilage was administrated p.o. by original liquid of 0.35ml /day/ mouse for two weeks.

Result: The vessels in tumor located areas were clearly found in the untreated control group. Almost all vessels disappeared in GCP-treated mice while other commercial products and shark cartilage did not show such effects．

Administration：GCP, Product A, & Product B were dissolved in a 10 % tap water solution and administrated, p.o. in the dosage of 0.35ml/day/mouse（1g/kg body weight). The liquid shark cartilage was administered p.o. with a solution of 0.35ml /day/mouse for a two week period.

Observation：Tumor growth curve, tumor weight.

Results: GCP significantly inhibited the tumor growth of S-180. Yet, shark cartilage, Product A or B did not shown this inhibitory effect.