15. Co-administration of AHCC and GCP

Studies have demonstrated that GCP’s main functions are in anti-angiogenesis and apoptosis induction of tumor cells and tissues. On the other hand, AHCC can increase immunoreactivity by up-regulating several cytokines such as IL-1, IL-2, IL-12, IFN-γ, TNF-α. In addition AHCC enhances the activities of macrophages, LAK and NK cells. Because both AHCC and GCP have shown anti-tumor activities, their synergistic anti-tumor effects were investigated in vitro and in vivo. Prostate cancer is one of the most challenging cancers to control in men. We used human prostate cancer cell lines-PC-3 as the target cell in the study.

PC-3 cell line was cultured in a 96 well plate in RPMI1640 medium containing 10% FBS with a cell number of 20,000 per ml medium for 24 hrs. Then, the samples were dissolved in DMSO (10%) and were added to the medium in the final concentrations shown in the figure. The control was added in the same volume of DMSO. After the cells were cultured for another 48 hrs, the growth of the cells was measured using MTT assay kit and the values shown in the figure are means of triple wells.

As seen in the Figure, the co-administration of AHCC and GCP showed a clear dose-dependent inhibitory effect on PC-3 tumor cells.